Absorbance is a fundamental concept in spectrophotometry, which is a technique used to measure the amount of light absorbed by a sample. It plays an essential role in various fields, including ...

Absorbance and transmittance are two essential parameters in the field of spectroscopy. They provide valuable information on how much light is absorbed and transmitted through a sample, respectively.

Dilute the dye to 0.2 g/L (Ci) with deionized water. Use the spectrophotometer to determine the maximum absorption wavelength and develop a “concentration-absorbance” curve using that value. Establish ...

An ELISA test should always be performed in replicates (duplicates or triplicates) so as to achieve enough number of samples to calculate the standard deviation and ...